Table 1. Analysis of reactivation frequency from ICP0- and TK promoter-marked cells following incubation at 43 °C for 2 h.
Latent ZsGreen-positive cells were plated at one positive cell per well. Remaining TG suspensions containing negative cells were plated separately. Reactivation was scored following the development of CPE within a well. Note: larger numbers of mouse TG were used to collect ICP0- and TK-marked cells to isolate these scarcer cell populations.
| Promoter | Total no. wells | Total no. wells reactivated | Reactivation (%) |
|---|---|---|---|
| CMVCre (1 mouse) | |||
| ZsGreen + | 72 | 9 | 12.5 |
| ICP0Cre (2 mice) | |||
| ZsGreen + | 48 | 2 | 4.2 |
| ZsGreen − | 48 | 30 | 62.5 |
| TKCre (4 mice) | |||
| ZsGreen + | 20 | 2 | 10 |
| ZsGreen − | 72 | 66 | 91.7 |