Table 4.

Precision (intra- and interday) and accuracy of the nine analytes

Analyte	Analyte concentration (μg/mL)	Intraday (n = 5)			Interday (n = 5)
		Calculated concentration (mean ± SD, μg/mL)	RSD (%)	Accuracy (%)	Calculated concentration (mean ± SD, μg/mL)	RSD (%)	Accuracy (%)
Homogentisic acid	2.78	2.82 ± 0.02	0.85	101.69	2.84 ± 0.01	0.16	102.12
	5.56	5.76 ± 0.04	0.75	103.61	5.60 ± 0.05	0.91	100.87
	11.11	11.37 ± 0.06	0.55	102.34	10.90 ± 0.08	0.75	98.12
Liquiritin	2.78	2.79 ± 0.01	0.25	100.60	2.81 ± 0.01	0.40	100.99
	5.56	5.69 ± 0.07	1.19	102.34	5.71 ± 0.01	0.13	102.81
	11.11	11.42 ± 0.03	0.24	102.82	11.44 ± 0.01	0.04	102.96
Naringin	2.78	2.87 ± 0.01	0.35	103.45	2.89 ± 0.01	0.25	104.12
	5.56	5.67 ± 0.03	0.61	102.08	5.63 ± 0.02	0.43	101.27
	11.11	11.49 ± 0.01	0.11	103.40	11.35 ± 0.01	0.09	102.14
Hesperidin	11.11	11.76 ± 0.03	0.27	105.86	11.71 ± 0.02	0.17	105.43
	22.22	23.46 ± 0.16	0.68	105.55	23.24 ± 0.10	0.41	104.57
	44.44	46.39 ± 0.19	0.40	104.38	46.36 ± 0.15	0.33	104.32
Neohesperidin	2.78	2.86 ± 0.02	0.58	103.08	2.88 ± 0.01	0.20	103.54
	5.56	5.69 ± 0.03	0.59	102.47	5.70 ± 0.02	0.38	102.67
	11.11	11.46 ± 0.02	0.15	103.10	11.32 ± 0.03	0.26	101.86
Liquiritigenin	2.78	2.90 ± 0.02	0.65	104.31	2.88 ± 0.01	0.31	103.73
	5.56	5.80 ± 0.01	0.13	104.48	5.74 ± 0.04	0.61	103.34
	11.11	11.60 ± 0.05	0.44	104.41	11.44 ± 0.01	0.04	102.92
Glycyrrhizin	8.33	8.45 ± 0.05	0.57	101.37	8.46 ± 0.01	0.15	101.50
	16.67	17.08 ± 0.01	0.09	102.50	16.96 ± 0.01	0.06	101.74
	33.33	33.70 ± 0.07	0.21	101.09	33.68 ± 0.07	0.20	101.03
6-Gingerol	2.78	2.85 ± 0.01	0.09	102.65	2.85 ± 0.01	0.20	102.53
	5.56	5.60 ± 0.02	0.33	100.84	5.62 ± 0.06	1.00	101.18
	11.11	11.40 ± 0.04	0.31	102.58	11.23 ± 0.03	0.30	101.06
Pachymic acid	5.56	5.61 ± 0.03	0.59	100.98	5.60 ± 0.02	0.42	100.88
	11.11	11.15 ± 0.05	0.41	100.38	11.20 ± 0.04	0.37	100.80
	22.22	22.56 ± 0.05	0.21	101.32	22.47 ± 0.05	0.22	101.11

RSD, relative standard deviation; SD, standard deviation.