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. 2014 Jun 13;4:5282. doi: 10.1038/srep05282

Figure 1. Changes in DNMTs, MBD2 and RNA Pol II binding at the leptin promoter in DIO mice.

Figure 1

Genomic DNA was purified from mouse epididymal fat and was immunoprecipitated with antibodies specific for DNMT1, DNMT3a, DNMT3b, MBD2 and RNA Pol II. The immunoprecipitated DNA was used in PCR reactions to detect the presence of leptin promoter DNA. Normal mouse IgG was used as a negative control. The ratio of the PCR signal from the protein ChIP DNA to the signal from the total genomic DNA (Input) is plotted as an estimation of the protein levels. The values are expressed as the mean ± SEM; n = 12 in each group. * compared to the control diet, P<0.05; # compared to the DIO diet, P<0.005.