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. 2014 Jun 13;4:5282. doi: 10.1038/srep05282

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Genomic DNA was purified from mouse epididymal fat and was immunoprecipitated with antibodies specific for methyl-H3K4, methyl-H3K9 and methyl-H3K27. The immunoprecipitated DNA was used in PCR reactions to detect the presence of leptin promoter DNA. Normal mouse IgG was used as a negative control. The ratio of the PCR signal from the protein ChIP DNA to the signal from the total genomic DNA (Input) is plotted as an estimation of the level of methy-H3K4, H3K9 and H3K27. The values are expressed as the mean ± SEM; n = 12 in each group. * compared to the control diet, P<0.005; ^# compared to the DIO diet, P<0.001.