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. 2014 Jun 5;4:5181. doi: 10.1038/srep05181

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(a) q-PCR analysis of Xist RNA in Ftx-deficient blastocysts. The ratios of the number of cDNA copies for Xist to that of cDNA copies for a housekeeping gene, β-actin, are indicated as expression levels. The bar graphs and lines show the mean ± standard deviation (SD) (n = 3). (b) RNA FISH analysis for Xist (red) expression in Ftx-deficient blastocysts. The nuclei of blastocysts were stained with DAPI (blue). Scale bar, 50 μm. (c) Summary of Xist RNA FISH analysis. Three wild-type (+_m/+_p) and Three Ftx-deficient (+_m/−_p) blastocysts were examined. Cells with 2 Xist signals were not observed. (d) Analysis of X-linked gene expression levels in Ftx-deficient blastocysts. No differences in the allelic expression patterns of X-linked genes between JF1 and C57BL/6N embryos were detected using RFLP. Uncropped images of the full-length gels are presented in Supplementary Figure S1.