Table 2. Primers used for PCR reactions.
Gene name | Primer | Primer Sequence | Expected Product Size (bp) | GenBank Accession no.* |
---|---|---|---|---|
blaCMY-2 | 585F | 5′- CAG ACG CGT CCT GCA ACC ATT AAA -3′ | 454a,1 | AB212086 |
1038R | 5′- TAC GTA GCT GCC AAA TCC ACC AGT -3′ | |||
675F | 5′- AGG GAA GCC CGT ACA CGT T -3′ | 64a,2 | ||
738R | 5′- GCT GGA TTT CAC GCC ATA GG -3′ | |||
blaCTX-M | CTX-M(F) | 5′ -ATGTGCAGYACCAGTAA- 3′ | 536b | AY143430_CTX-M-24 |
CTX-M(R) | 5′ -CCGCTGCCGGTYTTATC- 3′ | |||
tet(A) | tet(A)(F) | 5′ -GCTACATCCTGCTTGCCTTC- 3′ | 210c | X61367 |
tet(A)(R) | 5′ -CATAGATCGCCGTGAAGAGG- 3′ | |||
tet(B) | tet(B)(F) | 5′ -TTGGTTAGGGGCAAGTTTTG- 3′ | 659c | J01830 |
tet(B)(R) | 5′ -GTAATGGGCCAATAACACCG- 3′ | |||
16S rRNA | 1056F | 5′ - AAT GTT GGG TTA AGT CCC GCA ACG - 3′ | 400a,1 | EU014689 |
1456R | 5′ - ATG ATC ACA AAG TGG TAA GCG CCC - 3′ | |||
P201 | 5′- GAG GAA GGI GIG GAI GAC GT - 3′ | 216d,2 | ||
P1370 | 5′ - AGI CCC GIG AAC GTA TTC AC - 3′ |
aPrimer set used are from Alali et al.32
bPrimer set used are from Cottell et al.25
cPrimer set used are from Ng et al.49
dPrimer set used are from Steinman et al.50
1Broad range primers to generate template for the standard curve.
2Narrow range primers for sample quantification.
*Sequence used for primer design.