Table 2.
ORF prediction in LbFV genome
| ORF # | O | Start | Stop | Length (aa) | Hits found | Top Hit accession number | Description | e-value | Top hit species | Kingdom | Accession | Clan | Description | Start | End | Ind-e-value | Cond-e-value | TM | SP | struct. MdSGHV | struct. GpSGHV |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ORF1 | + | 214 | 1164 | 317 | 585 | W6JLD1_9POXV | Translation elongation factor eEF-3 like | 3.50E-34 | Anomala cuprea entomopoxvirus | Virus | PF14388.4 | n/a | Domain of unknown function (DUF4419) | 26 | 311 | 6.60E-45 | 4.00E-49 | ||||
| ORF2 | + | 1243 | 2247 | 335 | 0 | ||||||||||||||||
| ORF3 | + | 2314 | 2472 | 53 | 0 | ||||||||||||||||
| ORF4 | − | 2514 | 3530 | 339 | 0 | ||||||||||||||||
| ORF5 | + | 3767 | 5857 | 697 | 1 | A0A084WAW3_ANOSI | Nav1.4a | 5.70E-06 | Culex quinquefasciatus | Eukaryote | 2 | ||||||||||
| ORF6 | + | 5899 | 6450 | 184 | 0 | ||||||||||||||||
| ORF7 | + | 6455 | 7237 | 261 | 0 | ||||||||||||||||
| ORF8 | + | 7379 | 7633 | 85 | 0 | ||||||||||||||||
| ORF9 | + | 7721 | 8041 | 107 | 0 | ||||||||||||||||
| ORF10 | − | 8197 | 8475 | 93 | 2304 | C8AXE7_9CICH | TMO-4c4 (Fragment) | 1.30E-23 | Oreochromis niloticus | Eukaryote | |||||||||||
| ORF11 | − | 8838 | 11177 | 780 | 94 | B4MZD3_DROWI | Uncharacterized protein | 2.70E-17 | Lucilia cuprina | Eukaryote | PF02373.20 | CL0029 | JmjC domain, hydroxylase | 475 | 602 | 5.00E-09 | 3.10E-13 | ||||
| ORF12 | + | 11590 | 11769 | 60 | 0 | ||||||||||||||||
| ORF13 | − | 12479 | 14209 | 577 | 929 | A0A0P6K4V2_HETGA | V-type proton ATPase subunit a | 2.10E-20 | Fukomys damarensis | Eukaryote | PF02373.20 | CL0029 | JmjC domain, hydroxylase | 283 | 403 | 1.20E-10 | 7.40E-15 | ||||
| ORF14 | + | 15338 | 16237 | 300 | 169 | A0A0B5A143_9ABAC | Uncharacterized protein | 1.10E-21 | Pseudoplusia includens SNPV IE | Virus | PF02498.15 | n/a | BRO family, N-terminal domain | 97 | 195 | 5.50E-14 | 3.40E-18 | ||||
| ORF15 | + | 16558 | 17091 | 178 | 0 | ||||||||||||||||
| ORF16 | + | 17012 | 17185 | 58 | 0 | ||||||||||||||||
| ORF17 | + | 17615 | 17932 | 106 | 0 | ||||||||||||||||
| ORF18 | + | 17954 | 18655 | 234 | 0 | ||||||||||||||||
| ORF19 | + | 18662 | 19342 | 227 | 0 | ||||||||||||||||
| ORF20 | − | 19754 | 20902 | 383 | 0 | ||||||||||||||||
| ORF21 | − | 20974 | 21804 | 277 | 0 | ||||||||||||||||
| ORF22 | − | 21829 | 22842 | 338 | 0 | ||||||||||||||||
| ORF23 | + | 22941 | 23168 | 76 | 0 | ||||||||||||||||
| ORF24 | + | 23234 | 23683 | 150 | 0 | ||||||||||||||||
| ORF25 | − | 23838 | 24026 | 63 | 0 | ||||||||||||||||
| ORF26 | + | 24673 | 24882 | 70 | 0 | ||||||||||||||||
| ORF27 | + | 25506 | 26531 | 342 | 3883 | A0A182XPW4_ANOQN | Uncharacterized protein | 5.00E-100 | Anopheles christyi | Eukaryote | PF00653.19 | CL0417 | Inhibitor of Apoptosis domain | 11 | 78 | 3.50E-15 | 4.30E-19 | ||||
| PF00653.19 | CL0417 | Inhibitor of Apoptosis domain | 172 | 237 | 4.90E-16 | 6.00E-20 | |||||||||||||||
| PF13920.4 | CL0229 | Zinc finger, C3HC4 type (RING finger) | 290 | 334 | 1.10E-11 | 1.30E-15 | |||||||||||||||
| ORF28 | − | 26599 | 27075 | 159 | 0 | ||||||||||||||||
| ORF29 | − | 27068 | 27736 | 223 | 0 | ||||||||||||||||
| ORF30 | + | 27781 | 28251 | 157 | 0 | ||||||||||||||||
| ORF31 | − | 28266 | 28901 | 212 | 0 | ||||||||||||||||
| ORF32 | + | 29002 | 30687 | 562 | 0 | ||||||||||||||||
| ORF33 | + | 30976 | 32172 | 399 | 16 | Q9YVQ5_MSEPV | ORF MSV187 putative late transcription factor VLTF-2 homolog (Vaccinia A1L), similar to SW:P33814 | 9.20E-13 | Melanoplus sanguinipes entomopoxvirus | Virus | |||||||||||
| ORF34 | + | 32165 | 32380 | 72 | 0 | ||||||||||||||||
| ORF35 | + | 32481 | 33695 | 405 | 7 | Q9YVQ5_MSEPV | ORF MSV187 putative late transcription factor VLTF-2 homolog (Vaccinia A1L), similar to SW:P33814 | 6.50E-09 | Melanoplus sanguinipes entomopoxvirus | Virus | |||||||||||
| ORF36 | − | 33675 | 38513 | 1613 | 0 | ||||||||||||||||
| ORF37 | − | 38599 | 39297 | 233 | 106 | Q7T5L7_GVCL | Dbp | 3.20E-15 | Cryptophlebia leucotreta granulosis virus | Virus | PF00293.26 | CL0261 | NUDIX domain | 23 | 165 | 6.20E-09 | 3.80E-13 | ||||
| ORF38 | + | 39418 | 39630 | 71 | 0 | ||||||||||||||||
| ORF39 | − | 39643 | 39909 | 89 | 0 | ||||||||||||||||
| ORF40 | + | 39929 | 40141 | 71 | 0 | ||||||||||||||||
| ORF41 | − | 40381 | 40533 | 51 | 0 | ||||||||||||||||
| ORF42 | − | 40611 | 41324 | 238 | 0 | ||||||||||||||||
| ORF43 | + | 41497 | 42507 | 337 | 0 | ||||||||||||||||
| ORF44 | + | 43692 | 43880 | 63 | 0 | ||||||||||||||||
| ORF45 | + | 44031 | 44186 | 52 | 1 | A6YEY0_9NEOP | NADH- ubiquinone oxidoreductase chain 1 (Fragment) | 0.00018 | Operophtera brumata | Eukaryote | |||||||||||
| ORF46 | + | 44418 | 44576 | 53 | 0 | ||||||||||||||||
| ORF47 | + | 44612 | 44770 | 53 | 0 | ||||||||||||||||
| ORF48 | + | 45204 | 45365 | 54 | 0 | ||||||||||||||||
| ORF49 | − | 45626 | 45889 | 88 | 166 | W4XJ30_STRPU | Uncharacterized protein | 2.20E-06 | Strongylocentrotus purpuratus | Eukaryote | PF01712.17 | CL0023 | Deoxynucleoside kinase | 1 | 86 | 1.90E-11 | 1.20E-15 | ||||
| ORF50 | − | 45946 | 46284 | 113 | 120 | D9YXA4_9CRUS | NADH-ubiquinone oxidoreductase chain 2 (Fragment) | 4.60E-10 | Daphnia magna | Eukaryote | PF01712.17 | CL0023 | Deoxynucleoside kinase | 5 | 111 | 2.70E-16 | 1.70E-20 | ||||
| ORF51 | − | 46894 | 48393 | 500 | 0 | ||||||||||||||||
| ORF52 | − | 48380 | 49501 | 374 | 37 | A0A120HYA7_GHVS | Per-os infectivity factor 2-like protein | 5.30E-13 | Glossina hytrovirus | Virus | 1 | x | |||||||||
| ORF53 | − | 49589 | 49843 | 85 | 0 | ||||||||||||||||
| ORF54 | − | 49905 | 50240 | 112 | 2 | A0A0A1HB82_LINLA | Cytochrome c oxidase subunit 1 (Fragment) | 6.00E-13 | Strongylocentrotus purpuratus | Eukaryote | 2 | ||||||||||
| ORF55 | + | 50261 | 50857 | 199 | 0 | ||||||||||||||||
| ORF56 | − | 50854 | 51411 | 186 | 0 | ||||||||||||||||
| ORF57 | + | 51395 | 51547 | 51 | 0 | ||||||||||||||||
| ORF58 | + | 51528 | 55454 | 1309 | 3 | A0A0K1L634_9VIRU | ORF_007L | 6.30E-05 | Scale drop disease virus Iridoviridae | Virus | PF00136.19 | CL0194 | DNA polymerase family B | 599 | 880 | 2.30E-20 | 1.40E-24 | 2 | |||
| ORF59 | − | 55493 | 56143 | 217 | 0 | ||||||||||||||||
| ORF60 | − | 56177 | 57265 | 363 | 4 | R9XIV9_ASHAC | AaceriAGL139Wp | 4.70E-09 | Glossina hytrovirus | Virus | PF02450.13 | CL0028 | Lecithin:cholesterol acyltransferase | 57 | 351 | 1.60E-07 | 9.60E-12 | ||||
| ORF61 | − | 57490 | 59226 | 579 | 476 | B4JWM1_DROGR | GH22716 | 4.80E-78 | Drosophila melanogaster | Eukaryote | mucin | 1 | |||||||||
| ORF62 | − | 59414 | 59950 | 179 | 0 | ||||||||||||||||
| ORF63 | − | 60093 | 62282 | 730 | 153 | B0YLJ3_GHVS | p74 protein-like protein | 1.30E-68 | Glossina hytrovirus | Virus | x | x | |||||||||
| ORF64 | − | 62255 | 62407 | 51 | 0 | ||||||||||||||||
| ORF65 | + | 63240 | 63398 | 53 | 0 | ||||||||||||||||
| ORF66 | + | 63602 | 65116 | 505 | 1892 | B4NG60_DROWI | Uncharacterized protein | 1.30E-50 | Lucilia cuprina | Eukaryote | PF00653.19 | CL0417 | Inhibitor of Apoptosis domain | 123 | 189 | 2.40E-12 | 2.90E-16 | ||||
| PF00653.19 | CL0417 | Inhibitor of Apoptosis domain | 290 | 355 | 1.60E-14 | 2.00E-18 | |||||||||||||||
| PF13920.4 | CL0229 | Zinc finger, C3HC4 type (RING finger) | 396 | 442 | 2.30E-08 | 2.80E-12 | |||||||||||||||
| PF13920.4 | CL0229 | Zinc finger, C3HC4 type (RING finger) | 452 | 495 | 1.60E-06 | 2.00E-10 | |||||||||||||||
| ORF67 | − | 65213 | 66142 | 310 | 4 | A0A0U4AXZ3_9VIRU | Putative helicase | 2.30E-06 | Pithovirus sibericum | Virus | |||||||||||
| ORF68 | − | 66316 | 68253 | 646 | 0 | ||||||||||||||||
| ORF69 | + | 68261 | 68521 | 87 | 0 | ||||||||||||||||
| ORF70 | + | 68697 | 68873 | 59 | 0 | ||||||||||||||||
| ORF71 | − | 68975 | 69184 | 70 | 0 | ||||||||||||||||
| ORF72 | + | 69345 | 69665 | 107 | 0 | ||||||||||||||||
| ORF73 | + | 69662 | 70030 | 123 | 0 | ||||||||||||||||
| ORF74 | + | 70085 | 70495 | 137 | 0 | ||||||||||||||||
| ORF75 | + | 70641 | 70805 | 55 | 0 | ||||||||||||||||
| ORF76 | − | 70836 | 71072 | 79 | 0 | ||||||||||||||||
| ORF77 | − | 71685 | 71921 | 79 | 0 | ||||||||||||||||
| ORF78 | + | 72249 | 74279 | 677 | 5 | A0A0R3WJU6_HYDTA | Uncharacterized protein | 1.60E-09 | Glossina hytrovirus | Virus | 1 | x | |||||||||
| ORF79 | + | 74511 | 75440 | 310 | 0 | ||||||||||||||||
| ORF80 | + | 75437 | 76492 | 352 | 0 | ||||||||||||||||
| ORF81 | − | 76495 | 78105 | 537 | 5912 | B0YLU3_GHVS | Uncharacterized protein | 2.20E-18 | Glossina hytrovirus | Virus | PF00004.27 | CL0023 | ATPase associated with various activities (AAA) | 202 | 331 | 4.40E-14 | 2.70E-18 | x | x | ||
| ORF82 | + | 78323 | 78643 | 107 | 0 | ||||||||||||||||
| ORF83 | + | 78694 | 79995 | 434 | 0 | ||||||||||||||||
| ORF84 | − | 80036 | 80317 | 94 | 0 | ||||||||||||||||
| ORF85 | + | 81459 | 82106 | 216 | 77 | B2YG85_MHVB | Ac81-like protein | 6.70E-10 | Musca hytrovirus | Virus | PF05820.9 | n/a | Baculovirus protein of unknown function (DUF845) | 29 | 147 | 0.00015 | 9.10E-09 | 2 | x | ||
| ORF86 | + | 82084 | 82293 | 70 | 0 | ||||||||||||||||
| ORF87 | + | 82378 | 82908 | 177 | 0 | ||||||||||||||||
| ORF88 | + | 82908 | 83156 | 83 | 0 | ||||||||||||||||
| ORF89 | - | 83275 | 83892 | 206 | 2 | B6DZA6_9VIRU | Uncharacterized protein | 4.40E-107 | Leptopilina boulardi filamentous virus | Virus | 1 | ||||||||||
| ORF90 | + | 84156 | 85292 | 379 | 0 | ||||||||||||||||
| ORF91 | + | 85375 | 85536 | 54 | 0 | ||||||||||||||||
| ORF92 | + | 85846 | 90627 | 1594 | 2 | B0YLK7_GHVS | Per-os infectivity factor 2-like protein | 3.30E-18 | Glossina hytrovirus | Virus | 4 | x | |||||||||
| ORF93 | + | 90767 | 90979 | 71 | 0 | ||||||||||||||||
| ORF94 | + | 91062 | 91610 | 183 | 0 | ||||||||||||||||
| ORF95 | + | 91631 | 91834 | 68 | 1 | A0A061DCU0_BABBI | Uncharacterized protein | 0.00012 | Theileria annulata | Eukaryote | 1 | ||||||||||
| ORF96 | − | 91717 | 94863 | 1049 | 2 | B0YLT7_GHVS | Putative uncharacterized protein | 5.50E-28 | Glossina hytrovirus | Virus | x | ||||||||||
| ORF97 | + | 94936 | 95808 | 291 | 0 | x | x | ||||||||||||||
| ORF98 | + | 95820 | 96437 | 206 | 0 | ||||||||||||||||
| ORF99 | + | 96467 | 97387 | 307 | 0 | ||||||||||||||||
| ORF100 | − | 97479 | 97787 | 103 | 0 | ||||||||||||||||
| ORF101 | − | 97813 | 98703 | 297 | 1 | R9X889_ASHAC | Vacuolar ATPase assembly integral membrane protein VMA21 | 6.70E-06 | Musca hytrovirus | Virus | PF10553.7 | n/a | MSV199 domain | 23 | 101 | 5.40E-05 | 3.30E-09 | 1 | |||
| ORF102 | − | 99010 | 100092 | 361 | 0 | ||||||||||||||||
| ORF103 | − | 100137 | 100715 | 193 | 0 | ||||||||||||||||
| ORF104 | + | 100910 | 101581 | 224 | 0 | ||||||||||||||||
| ORF105 | + | 103681 | 104427 | 249 | 1 | A0A016TCB2_9BILA | Hexosyltransferase | 2.40E-06 | Caenorhabditis brenneri | Eukaryote | |||||||||||
| ORF106 | − | 104449 | 106506 | 686 | 138 | A0A0B7JV03_BIOOC | Occlusion-derived virus envelope protein | 1.10E-50 | Musca hytrovirus | Virus | PF08124.9 | CL0372 | Polysaccharide lyase family 8, N terminal alpha-helical domain | 56 | 283 | 1.30E-10 | 8.20E-15 | 2 | x | ||
| ORF107 | + | 106977 | 108854 | 626 | 1 | A0A158R6B8_9BILA | Uncharacterized protein | 0.00023 | Helobdella robusta | Eukaryote | |||||||||||
| ORF108 | + | 108899 | 110278 | 460 | 28 | G5AXB1_HETGA | Protein CASP | 2.10E-22 | Fukomys damarensis | Eukaryote |
Note.—The annotation was done using hmmer algorithm for protein (phmmer) and domain (hmmscan) searches.
O: Orientation; SP, signal peptide; TM, transmembrane domain; Struct. MdSGHV: homolog has been identified as a structural protein in MdSGHV; Struct. GpSGHV: homolog has been identified as a structural protein in GpSGHV.