Fig. 2.

RASSF5 sequence and structure. Human RASSF5 contains 418 residues involving the RA domain (residues 274–364) and SARAH (residues 366–413). (a) The sequence of N-terminal truncated RASSF5 protein with each domain structure embedded in a cartoon representation. In the sequence, hydrophobic/glycine, polar, positively charged, and negatively charged residues are colored black, green, blue, and red, respectively. Similarly, in the ribbon representation for the secondary structures, the same colors were used, except for the hydrophobic/glycine residues which are colored white. (b) Crystal structures of murine RA domain (PDB code: 3DDC) and human SARAH domain (PDB code: 4LGD) as a long straight α-helix defined in a coiled coil with MST2 SARAH domain. In the RA domain, the missing flexible loop (residues 254–278) was constructed by using the Modeller server.³⁰