Fig. 3.—

A-to-I editing in the genes SMAC_02537 and SMAC_04643 can be detected in the wild type, but not in developmental mutant pro1. PCR fragments derived from cDNA from samples grown for 5d under conditions allowing sexual development were sequenced by Sanger sequencing. Chromatograms from sequences around edited sites are shown for wild type and developmental mutant pro1. Below each site, the position within the gene and the percentage of edited sequence reads in the RNA-seq data from wild type protoperithecia are given. Editing of these sites was not observed in RNA-seq data from pro1 protoperithecia.