Fig. 5.—

Verification of RNA editing in P. confluens. Sixteen putative editing sites in five genes were analyzed by sequencing cDNA clones from sexually developing samples after 3d or 5d (8–14 cDNA clones for each sample). Genomic DNA was also sequenced and was found to be as expected in all cases (data not shown). (A) Editing sites were sorted from low to high editing frequency in the RNA-seq data, and the percentage of editing observed in the sequenced cDNA clones in the different samples is indicated for each site. (B) For each analyzed gene, the percentage of cDNAs with the indicated number of edited sites is shown for each of the conditions. The number of potential editing sites for each analyzed DNA fragment is given in square brackets after the gene name.