Table 1.
Predicted Effectors in the Version TwoSclerotiniasclerotiorum Genome (SsPEs) and Their Corresponding Version One Loci
| Version One ID | Version Two ID | Percent Identity | Version One Length | Version Two Length | Guyon et al. Prediction? | Version Two Length/Version One Length | Domains Predicted by InterProScan (prediction database|domain identifier|description|) | % AA Identity of Best Blast Hit | E-Value of Best Blast Hit |
|---|---|---|---|---|---|---|---|---|---|
| SS1G_09693 | Sscle01g000660 | 100 | 459 | 459 | 1 | ||| | 57.534 | 2.34e−050 | |
| SS1G_02068 | Sscle01g003850 | 100 | 565 | 565 | 1 | ||| | 55.621 | 2.7e−056 | |
| SS1G_01974 | Sscle01g004620 | 100 | 465 | 558 | 1.2 | ||| | 86.607 | 5.13e−052 | |
| SS1G_01867 | Sscle01g005390 | 100 | 490 | 490 | 1 | ||| | 90.741 | 3.46e−059 | |
| SS1G_01754 | Sscle01g006330 | 100 | 2,266 | 532 | 0.234774934 | ||| | 65.035 | 9.79e−055 | |
| SS1G_01427 | Sscle01g008940 | 100 | 1,512 | 1,512 | 1 | Pfam|PF00024|PAN domain| | 69.421 | 3.37e−168 | |
| SS1G_01426 | Sscle01g008950 | 100 | 1,079 | 1,079 | 1 | ||| | 82.158 | 2.12e−119 | |
| SS1G_01287 | Sscle01g009960 | 100 | 624 | 624 | 1 | ||| | 29.353 | 3.44e−011 | |
| SS1G_01214 | Sscle01g010490 | 100 | 404 | 404 | 1 | Pfam;SUPERFAMILY;Gene3D|PF06766;SSF101751; G3DSA:3.20.120.10|Fungal hydrophobin;;| | 63.265 | 9.41e−039 | |
| SS1G_12778 | Sscle02g012940 | 100 | 282 | 282 | 1 | ||| | — | — | |
| SS1G_04766 | Sscle02g014280 | 100 | 881 | 348 | 0.395005675 | Pfam;SUPERFAMILY;Gene3D|PF00190;SSF51182; G3DSA:2.60.120.10|Cupin;;| | 84.404 | 2.38e−059 | |
| SS1G_04618 | Sscle02g015390 | 100 | 468 | 468 | 1 | ||| | 72.611 | 1.13e−079 | |
| SS1G_04155 | Sscle02g019000 | 100 | 1,139 | 1,139 | 1 | ||| | 77.551 | 1.96e−149 | |
| SS1G_13012 | Sscle02g021780 | 100 | 755 | 838 | 1.109933775 | ProSiteProfiles;Gene3D;SUPERFAMILY; Pfam|PS50059;G3DSA:3.10.50.40;SSF54534; PF00254|FKBP-type peptidyl-prolyl cis-trans isomerase domain profile.;;;FKBP-type peptidyl-prolyl cis-trans isomerase| | 90.909 | 3.64e−112 | |
| SS1G_01032 | Sscle03g022550 | 100 | 1,178 | 1,178 | 1 | ||| | 79.894 | 0 | |
| SS1G_01003 | Sscle03g022790 | 100 | 450 | 450 | 1 | Gene3D;Pfam|G3DSA:3.20.120.10;PF06766|;Fungal hydrophobin| | 34.653 | 1.63e−012 | |
| SS1G_13371 | Sscle03g031910 | 100 | 880 | 880 | x | 1 | ||| | 61.404 | 7.12e−083 |
| SS1G_02522 | Sscle04g035160 | 100 | 897 | 897 | 1 | ||| | 87.9 | 5.62e−169 | |
| SS1G_02700 | Sscle04g036550 | 100 | 276 | 610 | 2.210144928 | ||| | 70.27 | 7.9e−081 | |
| SS1G_03057 | Sscle04g039210 | 100 | 484 | 484 | 1 | Pfam;Gene3D|PF03966;G3DSA:2.20.25.10| Trm112p-like protein;| | 96.85 | 8.66e−083 | |
| SS1G_03080 | Sscle04g039420 | 100 | 852 | 852 | x | 1 | Pfam;PIRSF;Coils|PF05630;PIRSF029958;Coil|Necrosis-inducing protein (NPP1);;| | 82.114 | 5.47e−147 |
| SS1G_14237 | Sscle04g040080 | 100 | 1,186 | 1,186 | 1 | Pfam|PF11327|Protein of unknown function (DUF3129)| | 82.4 | 9e−144 | |
| SS1G_12123 | Sscle05g041050 | 100 | 1,365 | 620 | 0.454212454 | ||| | 69.565 | 4.06e−075 | |
| SS1G_06068 | Sscle05g045060 | 100 | 352 | 352 | 1 | ProSiteProfiles|PS51257|Prokaryotic membrane lipoprotein lipid attachment site profile.| | 67.742 | 2.5e−035 | |
| SS1G_05939 | Sscle05g046060 | 100 | 264 | 264 | 1 | ProSiteProfiles|PS51257|Prokaryotic membrane lipoprotein lipid attachment site profile.| | 74.713 | 4.11e−041 | |
| SS1G_05938 | Sscle05g046070 | 100 | 639 | 639 | x | 1 | ||| | 58.772 | 1.7e−045 |
| SS1G_07491 | Sscle06g048920 | 100 | 709 | 526 | 0.741889986 | ||| | 75.51 | 5.86e−078 | |
| SS1G_07320 | Sscle06g050100 | 100 | 930 | 930 | 1 | SUPERFAMILY;TIGRFAM;PRINTS;PRINTS;PRINTS;PRINTS; PRINTS;Gene3D;SMART;ProSiteProfiles;Pfam; SMART|SSF52540;TIGR00231;PR00328;PR00328; PR00328;PR00328;PR00328;G3DSA:3.40.50.300;SM00178; PS51422;PF00025;SM00177|;small_GTP: small GTP-binding protein domain;GTP-binding SAR1 protein signature;GTP-binding SAR1 protein signature; GTP-binding SAR1 protein signature;GTP-binding SAR1 protein signature;GTP-binding SAR1 protein signature;;Sar1p-like members of the Ras-family of small GTPases;small GTPase SAR1 family profile.;ADP-ribosylation factor family;ARF-like small GTPases; ARF, ADP-ribosylation factor| | 99.471 | 8.88e−135 | |
| SS1G_07230 | Sscle06g050820 | 100 | 298 | 298 | 1 | ||| | 36.364 | 2.1 | |
| SS1G_07027 | Sscle06g052360 | 100 | 710 | 710 | 1 | ||| | 87.931 | 1.17e−109 | |
| SS1G_12482 | Sscle06g054400 | 100 | 797 | 797 | 1 | ||| | 73.82 | 1.5e−114 | |
| SS1G_12431 | Sscle06g054810 | 100 | 939 | 616 | 0.656017039 | ||| | 45.37 | 3.47e−022 | |
| SS1G_12365 | Sscle06g055280 | 100 | 843 | 843 | + | 1 | ||| | 67.606 | 1.84e−096 |
| SS1G_03381 | Sscle07g057000 | 100 | 1,841 | 2,124 | 1.153720804 | ||| | 61.029 | 1.69e−043 | |
| SS1G_11673 | Sscle07g061770 | 100 | 506 | 506 | 1 | SUPERFAMILY;ProSiteProfiles;Gene3D|SSF57414;PS50948; G3DSA:3.50.4.10|;PAN/Apple domain profile.;| | 65.625 | 3.07e−057 | |
| SS1G_11693 | Sscle07g061960 | 100 | 850 | 850 | + | 1 | ||| | 67.606 | 1.84e−096 |
| SS1G_11706 | Sscle07g062060 | 100 | 297 | 614 | 2.067340067 | ||| | 82.639 | 4.75e−082 | |
| SS1G_05103 | Sscle08g064180 | 100 | 520 | 520 | 1 | Coils|Coil‖ | 66.99 | 1.51e−042 | |
| SS1G_05152 | Sscle08g064590 | 100 | 529 | 529 | 1 | ||| | 74.051 | 2.25e−055 | |
| SS1G_05569 | Sscle08g067710 | 100 | 498 | 498 | 1 | ||| | 46.914 | 4e−035 | |
| SS1G_14184 | Sscle08g068200 | 100 | 1,560 | 1,350 | x | 0.865384615 | ProSitePatterns;SMART;ProSiteProfiles;ProSitePatterns; SUPERFAMILY;SUPERFAMILY;ProSitePatterns;ProSiteProfiles; SUPERFAMILY;ProSiteProfiles;Gene3D;Gene3D;SMART; SMART;SMART;Pfam;Gene3D;Gene3D;ProSitePatterns; ProDom;Pfam;SUPERFAMILY|PS00026;SM00236; PS51164;PS00026;SSF57016;SSF57016;PS00562;PS50941; SSF57016;PS50941;G3DSA:3.30.60.10;G3DSA:3.30.60.10; SM00270;SM00270;SM00270;PF00187;G3DSA:3.30.60.10; G3DSA:3.30.60.10;PS00026;PD001821;PF00734; SSF57180|Chitin recognition or binding domain signature.;Fungal-type cellulose-binding domain;CBM1 (carbohydrate binding type-1) domain profile.;Chitin recognition or binding domain signature.;;;CBM1 (carbohydrate binding type-1) domain signature.;Chitin-binding type-1 domain profile.;;Chitin-binding type-1 domain profile.;;;Chitin binding domain;Chitin binding domain;Chitin binding domain;Chitin recognition protein;;;Chitin recognition or binding domain signature.;DEGRADATION HYDROLASE GLYCOSIDASE CELLULOSE METABOLISM CARBOHYDRATE POLYSACCHARIDE PRECURSOR SIGNAL I;Fungal cellulose binding domain;| | 34.759 | 4.64e−014 |
| SS1G_10892 | Sscle09g069090 | 100 | 576 | 576 | 1 | Pfam|PF12296|Hydrophobic surface binding protein A| | 38.15 | 1.68e−028 | |
| SS1G_03897 | Sscle09g072630 | 100 | 481 | 481 | 1 | ||| | 64.384 | 9.4e−025 | |
| SS1G_03721 | Sscle09g074030 | 100 | 869 | 869 | + | 1 | ||| | 61.404 | 3.39e−084 |
| SS1G_08128 | Sscle10g074920 | 100 | 444 | 444 | 1 | ||| | 83.81 | 3.41e−047 | |
| SS1G_08163 | Sscle10g075140 | 99.49 | 393 | 395 | 1.005089059 | ||| | 66.667 | 1.75e−033 | |
| — | Sscle10g076600 | — | — | — | — | Pfam;SUPERFAMILY|PF07249;SSF50685|Cerato-platanin;| | 87.417 | 3.34e−085 | |
| SS1G_13851 | Sscle10g080580 | 100 | 441 | 275 | 0.623582766 | ||| | 49.231 | 4.2e−010 | |
| SS1G_08088 | Sscle11g081020 | 100 | 159 | 159 | 1 | ||| | — | — | |
| SS1G_07837 | Sscle11g082970 | 100 | 1,012 | 1,012 | 1 | ||| | 50.968 | 2.64e−051 | |
| SS1G_07613 | Sscle11g084720 | 100 | 628 | 628 | 1 | Pfam;Gene3D;SUPERFAMILY;SMART|PF02221; G3DSA:2.70.220.10;SSF81296;SM00737|ML domain;;;Domain involved in innate immunity and lipid metabolism.| | 86.628 | 3.87e−107 | |
| SS1G_11151 | Sscle12g087960 | 100 | 471 | 471 | 1 | ||| | 33.758 | 5.21e−017 | |
| SS1G_11085 | Sscle12g088530 | 100 | 772 | 772 | 1 | ||| | 47.573 | 1.37e−055 | |
| SS1G_11065 | Sscle12g088660 | 100 | 636 | 636 | 1 | ||| | 40.385 | 3.4 | |
| SS1G_11928 | Sscle12g090380 | 100 | 159 | 159 | 1 | ||| | — | — | |
| — | Sscle13g094760 | — | — | — | — | Pfam|PF10270|Membrane magnesium transporter| | 89.041 | 1.21e−090 | |
| SS1G_06729 | Sscle13g094920 | 100 | 802 | 802 | 1 | Pfam;ProSiteProfiles;Coils;SUPERFAMILY|PF01105;PS50866; Coil;SSF101576|emp24/gp25L/p24 family/GOLD;GOLD domain profile.;;| | 96.552 | 5.94e−145 | |
| SS1G_06763 | Sscle13g095230 | 100 | 709 | 709 | 1 | ||| | 44.624 | 4.16e−039 | |
| SS1G_14379 | Sscle13g097000 | 100 | 843 | 843 | + | 1 | ||| | 68.075 | 3.96e−097 |
| SS1G_08669 | Sscle14g098710 | 100 | 213 | 1,055 | 4.953051643 | Gene3D;SUPERFAMILY;Pfam|G3DSA:1.10.1280.10;SSF48056; PF00264|;;Common central domain of tyrosinase| | 68.705 | 7.45e−099 | |
| SS1G_08706 | Sscle14g098920 | 100 | 405 | 405 | 1 | SUPERFAMILY;Gene3D;Pfam|SSF50685;G3DSA:2.40.40.10; PF03330|;;Rare lipoprotein A (RlpA)-like double-psi beta-barrel| | 88.806 | 6.08e−081 | |
| SS1G_08892 | Sscle14g100310 | 100 | 908 | 908 | 1 | ||| | 65.845 | 8.4e−123 | |
| SS1G_13394 | Sscle15g102390 | 100 | 587 | 587 | 1 | ||| | 58.757 | 2.72e−059 | |
| SS1G_09420 | Sscle15g105160 | 100 | 528 | 528 | 1 | ||| | 77.586 | 1.6e−095 | |
| SS1G_09288 | Sscle15g106080 | 100 | 594 | 594 | 1 | ||| | 33.333 | 2.32e−019 | |
| SS1G_09150 | Sscle15g107190 | 100 | 648 | 648 | 1 | ||| | 36.782 | 0.001 | |
| SS1G_10104 | Sscle16g107730 | 100 | 880 | 880 | + | 1 | ||| | 61.842 | 3.08e−084 |
| SS1G_10129 | Sscle16g107890 | 100 | 333 | 333 | 1 | ||| | 46.032 | 0.026 | |
| SS1G_14320 | Sscle16g111080 | 100 | 483 | 483 | 1 | Pfam;ProSiteProfiles;Gene3D;SMART;SUPERFAMILY|PF01817; PS51168;G3DSA:1.20.59.10;SM00830;SSF48600|Chorismate mutase type II;Chorismate mutase domain profile.;;Chorismate mutase type II;| | 58.904 | 4.27e−048 | |
| — | Sscle16g111300 | — | — | — | — | ||| | 68.075 | 3.96e−097 |
Note.—In column six, a “x” indicates an “effector-like” prediction in Guyon et al., (2014), and a “+” indicates a sequence in the six-gene family paralogous to Sscle03g031910 (previously SS1G_13371) identified in Guyon et al.