Table 1.

PCR Sanger Sequencing Results vs. lobSTR Results for Selected STRs

						UMD3.1		PCR (bp)		lobSTR (bp)
No	STR	Chr	Begin	End	Motif	(bp)	Animal	A1	A2	A1	A2	Call	Coverage	Q score
1	BM1818	23	39,294,224	39,294,249	GT	37	Blackstar	33	37	33	33	P	4	0.69
2							Elevation	35	37	37	37	P	10	2.39
3							Ivanhoe	33	37	37	37	P	2	0.30
4							Chairman	33	37	33	37	Y	30	0.00
5	BM1824	1	132,498,006	132,498,034	CA	29	Blackstar	29	35	33	35	P	4	2.35
6							Elevation	29	35	27	35	P	10	2.39
7	BM2113	2	127,591,877	127,591,917	AC	42	Elevation	26	48	28	38	N	14	5.52
8							Blackstar	28	36	28	40	P	6	3.88
9	ETH10	5	56,657,954	56,657,996	CA	41	Elevation	39	43	37	43	P	6	4.09
10	ETH152	5	114,885,382	114,885,416	TG	37	Blackstar	33	35	35	35	P	14	4.17
11							Elevation	33	35	35	35	P	6	1.79
12	ETH225	9	10,858,165	10,858,199	CA	34	Elevation	32	34	30	32	N	8	0.69
13							Blackstar	26	38	38	38	P	2	0.25
14	ETH3	19	56648417	56648461	AC	46	Blackstar	42	52	44	52	P	6	6.00
15	HAUT27	26	29,127,336	29,127,370	GT	39	Elevation	29	41	31	31	N	2	0.37
16	ILSTS006	7	96,709,240	96,709,279	TG	43	Elevation	39	43	35	41	N	6	4.12
17							Blackstar	39	43	43	43	P	2	0.37
18	INRA023	3	33,011,005	33,011,044	CA	43	Chairman	31	37	31	31	P	28	3.62
19							Blackstar	31	35	31	35	Y	6	2.82
20							Elevation	35	39	35	39	Y	6	2.87
21							Ivanhole	31	39	31	39	Y	18	4.06
22	INRA037	10	76,365,534	76,365,555	TG	32	Blackstar	31	38	31	31	P	8	1.94
23	INRA063	18	40,699,867	40,699,892	GT	35	Blackstar	27	27	27	27	Y	4	0.80
24	STR_chr8	8	38,693,251	38,693,297	ACT	46	Blackstar	39	39	39	39	Y	4	3.11
25							Elevation	39	39	39	39	Y	14	3.74
26	STR_chr7	7	2,965,462	2,965,506	ATCC	44	Blackstar	44	44	44	44	Y	4	1.23
27							Elevation	44	48	44	48	Y	14	5.70
28	STR_chr10	10	37,414,396	37,414,434	ATCC	38	Blackstar	38	38	38	38	Y	2	0.70
29							Elevation	38	38	38	38	Y	10	3.01
30	STR_chr16	16	81,503,345	81,503,411	ATCC	66	Blackstar	30	66	30	66	Y	4	big
31							Elevation	30	30	30	30	Y	14	4.21
32	STR_chr19	19	46,403,668	46,403,701	ATCC	33	Blackstar	33	37	33	37	Y	12	big
33							Elevation	33	33	33	33	Y	10	3.01
34	STR_chr24	24	34,465,896	34,465,926	AGAT	30	Blackstar	30	30	30	30	Y	2	0.70
35							Elevation	26	26	30	30	N	8	2.41

Note.—Y: Both platforms agree. P: lobSTR reported only one allele out of two. N: lobSTR reported an allele that does not exist.