Table 1.
Nucleotidic and Amino Acid Compositions (Percentages Based on Direct Counts) of Alvinellid Coding Sequences and Translated Proteins Obtained from the Six Transcriptome Assemblies
| Species | A. pompejana# | A. caudata# | P. sulfincola# | P. fijiensis# | P. grasslei § | P. pandorae § |
|---|---|---|---|---|---|---|
| Nucleic acid content | ||||||
| Total GC% | 44.42 | 44.58 | 43.75 | 44.85 | 44.26 | 44.17 |
| GC12% | 44.90 | 44.80 | 44.90 | 45.00 | 44.70 | 44.60 |
| GC3% | 43.55 | 44.05 | 41.51 | 44.64 | 43.47 | 43.32 |
| Purine Load | 101.82 | 102.52 | 105.20 | 103.98 | 104.23 | 103.90 |
| rDNA-18S | 52.22 | 52.20 | 51.92 | 51.76 | 51.71 | 51.10 |
| Charged content | 27.13 *** | 27.04 * | 27.08 * | 27.36 *** | 26.85** | 26.70** |
| D (Asp) | 6.08 | 6.11 | 5.97*** | 6.06*** | 6.62 ** | 6.87 ** |
| E (Glu) | 7.25 | 7.07 | 7.43 ** | 7.27 *** | 6.69** | 6.71 |
| H (His) | 2.46 | 2.34 | 2.22 | 2.32 | 2.37** | 2.25 |
| K (Lys) | 6.62 | 6.85* | 6.45 | 6.51 | 6.55 | 6.43 |
| R (Arg) | 4.72 | 4.67 | 5.01 *** | 5.20 *** | 4.62 | 4.44 |
| Polar content | 29.99* | 29.84* | 30.18* | 29.55* | 31.17 ** | 31.13 ** |
| Q (Gln) | 4.08 | 4.09 | 4.19 | 4.04 | 4.24 | 4.09 |
| G (Gly) | 3.28 | 3.28 | 3.10 | 2.97* | 3.37 | 3.57 |
| N (Asn) | 5.08 | 5.07 | 5.31 | 5.28 | 5.29 | 5.17 |
| P (Pro) | 2.55 ** | 2.62 ** | 2.48 ** | 2.56 ** | 2.24 | 2.12 |
| S (Ser) | 8.08** | 8.13** | 8.27** | 8.05* | 9.19 *** | 9.48 *** |
| T (Thr) | 6.92* | 6.65 | 6.83 | 6.65 | 6.84 | 6.70 |
| Aliphatic content | 36.96 ** | 37.17 *** | 36.65 * | 36.93 ** | 36.21* | 36.39* |
| A (Ala) | 7.86 *** | 8.11 *** | 7.90 *** | 8.42 *** | 6.99** | 7.15* |
| C (Cys) | 1.42* | 1.35 | 1.36 | 1.29 | 1.37 | 1.17 |
| I (Ile) | 8.40 | 8.56 | 8.00 | 8.38 | 8.38 | 8.85* |
| L (Leu) | 6.98** | 6.90** | 6.96* | 6.69* | 6.63 | 6.68 |
| M (Met) | 3.42 | 3.50 | 3.20 | 3.22 | 3.59 *** | 3.98 *** |
| V (Val) | 8.88 | 8.75 | 9.23 | 8.93 | 9.25 | 8.56 |
| Aromatic content | 5.92 | 5.97 | 6.13 * | 6.15 ** | 5.76 | 5.81 |
| F (Phe) | 3.12 | 3.08 | 3.25 | 3.29 | 3.19 | 3.32 |
| W (Trp) | 0.30 | 0.31 | 0.33 | 0.32 * | 0.27 | 0.29 |
| Y (Tyr) | 2.50 | 2.58 * | 2.55 | 2.54 * | 2.30* | 2.20* |
Note.—All frequencies of each species were compared against either “cold” species (§) or “hot” species (#) according to an a priori ecological sign-test. Bold results discriminated hot from cold lineages.
Level of significance associated with the a priori ecological sign-test,
*
P < 0.05,
**
P < 0.01,
***
P < 0.001.