Table 2. Results of LC–HRAM–MS analysis of ‘RG’ and ‘M9’ stem tissue from grafted trees.
| Annotation | Type | Metabolite | 'RG' | 'M9' | FC 'M9'/'RG' |
|---|---|---|---|---|---|
| Arginine | Amino acid | 0.58 min: 521.326 m/z | 35.0 | 355.5 | 10.16 |
| Eriodictyol-hexoside | Flavonoid | 5.35 min: 449.109 m/z | 142.2 | 952.6 | 6.70 |
| Eriodictoyl | Flavonoid | 5.35 min: 287.059 m/z | 74.6 | 470.7 | 6.31 |
| Phloretin coumarylglucoside (?) | Flavonoid | 11.09 min: 581.162 m/z | 118.9 | 514.1 | 4.33 |
| Isorhamnetin ?-rhamnoside | Flavonoid | 7.04 min: 461.109 m/z | 167.7 | 490.0 | 2.92 |
| Procyanidin tetramer | Flavonoid | 4.41 min: 1153.258 m/z | 31.3 | 71.7 | 2.29 |
| Quercetin ?-rhamnoside | Flavonoid | 6.90 min: 447.093 m/z | 77.9 | 173.9 | 2.23 |
| p-coumaryl quinic acid | Phenolic acid | 4.01 min: 337.095 m/z | 616.4 | 1362.9 | 2.21 |
| Frag-p-coumaryl quinic acid | Phenolic acid | 4.01 min: 173.048 m/z | 145.2 | 302.3 | 2.08 |
| Phloretin-2'-O-xyloglucoside | Flavonoid | 6.10 min: 567.170 m/z | 368.5 | 726.3 | 1.97 |
| Kaempferol-?-pentoside | Flavonoid | 6.97 min: 417.084 m/z | 129.6 | 254.6 | 1.96 |
| Procyanidin B2 | Flavonoid | 3.42 min: 577.133 m/z | 262.6 | 450.5 | 1.72 |
| Procyanidin dimer (B5?) | Flavonoid | 5.37 min: 577.132 m/z | 69.8 | 111.3 | 1.60 |
| Kaempferol-?-pentoside | Flavonoid | 6.71 min: 417.084 m/z | 84.4 | 134.5 | 1.59 |
| Procyanidin trimer | Flavonoid | 4.17 min: 865.188 m/z | 224.5 | 347.4 | 1.55 |
| Epicatechin | Flavonoid | 3.83 min: 289.074 m/z | 1281.6 | 1948.1 | 1.52 |
| Kaempferol 3-rhamnoside | Flavonoid | 7.17 min: 431.098 m/z | 355.3 | 506.2 | 1.42 |
| Mearnsetin-glycoside | Flavonoid | 6.29 min: 477.103 m/z | 2155.5 | 2949.5 | 1.37 |
| Quercetin 3-arabinoside adduct | Flavonoid | 6.18 min: 867.152 m/z | 119.8 | 158.5 | 1.32 |
| Quercetin 3-xyloside | Flavonoid | 5.89 min: 433.078 m/z | 942.4 | 1189.5 | 1.26 |
| Quercetin 3-rhamnoside | Flavonoid | 6.35 min: 447.093 m/z | 3542.5 | 4338.7 | 1.22 |
| Quercetin 3-arabinoside | Flavonoid | 6.23 min: 433.078 m/z | 1554.4 | 1867.8 | 1.20 |
| Phloretin ?-pentoside | Flavonoid | 7.53 min: 405.120 m/z | 95.0 | 109.5 | 1.15 |
| Myricetin 3-pentoside | Flavonoid | 4.65 min: 449.073 m/z | 135.2 | 80.4 | −1.68 |
| Sucrose(quinic) adduct | 0.67 min: 533.171 m/z | 313.0 | 175.1 | −1.79 | |
| Quercetin 3-galactoside | Flavonoid | 5.54 min: 463.088 m/z | 389.3 | 202.7 | −1.92 |
| Quinic acid | Organic acid | 0.67 min: 191.059 m/z | 1069.8 | 447.0 | −2.39 |
| Oxo-dihydroxy-urs-12-ene-28-oic acid | Terpene | 14.92 min: 485.326 m/z | 3465.1 | 495.2 | −7.00 |
| Trihydroxy-urs-12-ene-28-oic acid | Terpene | 14.05 min: 487.341 m/z | 689.1 | 94.4 | −7.30 |
| Phloretin coumarylglucoside (?) | Flavonoid | 9.74 min: 581.166 m/z | 640.8 | 38.1 | −16.83 |
Abbreviations: LC–HRAM–MS, liquid chromatography–high-resolution accurate mass–mass spectrometry; M9, Malling 9; RG, Royal Gala.
Values are the means of two technical replicates of each individual and six biological replicates each of ‘RG’ and ‘M9’. The liquid chromatography retention time and accurate mass (m/z) for each metabolite is given. Concentrations are in units per mg dry weight, FC indicates fold change of 'M9' relative to 'RG'. The annotation is based on accurate mass and chemical formula calculations and authentic standards when available. The question marks indicate that a clear annotation could not be made.