Table 2. The effect of FBLN1C peptides on cell mitochondrial activity in fibroblasts and ASM cells.
| Cell type | Peptides | FBLN1C | hFN | |||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | ||
| Fibroblast N = 4 | ↑ | ---- | ---- | ---- | ---- | ---- | ---- | ↑ |
| ASM cell N = 5 | ↑ | ---- | ---- | ---- | ---- | ---- | ---- | ↑ |
Lung fibroblasts and airway smooth muscle (ASM) cells were seeded on precoated wells for 3 days and incubated with MTT for 4 hrs. MTT formazan was dissolved in 10% SDS and the intensity of blue colour detected using a SpectraMax M2 plate reader. Data were collected from N = 4 for fibroblast, 3/4 control, 1/4 chronic obstructive pulmonary disease (COPD); and N = 5 for ASM cell, 4/5 control, 1/5 COPD. FBLN1C1-7, fibulin1C peptide 1-7; hFN, human fibronectin. The concentrations of FBLN1C1-7 and hFN, used for experiments, were 3 and 10 μg/ml. ↑, P < 0.05 significantly different compared to PBS; ---- no change compared to PBS; repeated One-way ANOVA with Dunnett's multiple comparison test was performed.