Figure 1. Knockdown of Rab27A in RAW-D derived OCLs after stimulation with RANKL.

(a) Knockdown efficiency was measured by mRNA levels of Rab27A. After incubation with 10 p mol siRNA for 24 h, cells were cultured for additional for 5 days in the presence of RANKL (50ng/mL). (b) RAW-D cells were transfected with either a control siRNA or a siRNA specific for Rab27A. After stimulation with RANKL (50 ng/mL) for 72 h, OCLs were analyzed by TRAP-staining. (c) Total nucleus number of TRAP positive multinucleated OCLs, but not TRAP negative mononucleated cells following a 72 h culture, was counted per viewing field. ** P < 0.01. (D) The number of TRAP-positive multinucleated OCLs per viewing field was counted. Bar: 50 μm.