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. 2015 Apr 16;5:9620. doi: 10.1038/srep09620

Figure 4. Comparison of signaling levels of OCLs from wild-type and ashen mice.

Figure 4

(a) BMMs from wild-type and ashen mice were pre-incubated for 2 h in serum free media in the absence of M-CSF. After M-CSF addition, the cells were incubated for the indicated times, and consequently harvested. The same protein amounts of cell lysates were subjected to SDS-PAGE followed by western blotting with antibodies against p-Erk, p-p38, p-Src, p-Akt, and GAPDH. (b) BMMs from wild-type and ashen mice were pre-incubated for 2 h in serum free media in the absence of RANKL. After adding RANKL, the cells were incubated for the indicated times, consequently harvested. The same protein amounts of cell lysates were subjected to SDS-PAGE followed by western blotting with antibodies to p-Erk, p-p38, p-Akt, and GAPDH.