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ReAsH fluorescent lifetimes and the effect of solvent viscosity support a rotamer-restricted model for fluorogenicity. (A) The fluorescence decay of ReAsH-EDT2 and ReAsH-MBP-C4 as a function of time. Wavelength-dependent fluorescence emission of 450 μM (B) resorufin or (C) ReAsH-EDT2 in 100 mM NaOH at room temperature in the presence of the indicated amounts of glycerol. (D) Plot of the % change in the maximum emission of resorufin or ReAsH-EDT2 as a function of % added glycerol.
