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. 2017 Apr 5;12(4):e0174976. doi: 10.1371/journal.pone.0174976

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© 2017 Gates et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) An 11-week placenta sample was stained with rabbit anti-PRR15 antibody, or (B) with the rabbit anti-PRR15 antibody that was preadsorbed with recombinant PRR15 as a negative control. Six-week placenta was used to co-localize PRR15 (green fluorescence) with KRT7 (red fluorescence; trophoblast marker; C, E, G) and with CGB (red fluorescence; syncytiotrophoblast marker; D, F, H). Collectively, these data verify that PRR15 is predominantly localized to the nucleus of both cytotrophoblasts and syncytiotrophoblasts of the human placenta. Magnification bars represent: 20 μm for A, B, E, F, G and H; 50 μm for C and D.