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. 2017 Apr 5;12(4):e0174976. doi: 10.1371/journal.pone.0174976

Fig 5. Apoptosis increases in PRR15-deficient ACH-3P cells.

Fig 5

(A) Caspase 3/7 and 8 activity was measured using Caspase-Glo Reagent. Luminescence values were normalized to protein concentration in each well. * indicates p<0.05 in Student’s t-test. (B) Annexin V staining was quantified by flow cytometry. 7-AAD(-), AnnV(-) indicates cells that were not positive for either stain; 7-AAD(+) indicates necrotic cells; AnnV(+) indicates early apoptotic cells; 7-AAD(+), AnnV(+) indicates late apoptotic and necrotic cells. * indicates p<0.05, and ** indicates p<0.01 in Student’s t-test.