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Tissues in all groups were equilibrated for 30–40 min at 37°C in aerobic conditions. Muscle tissues were either maintained under aerobic conditions (AC) for the entire experimental period or subjected to 90 min of ischemia followed by 120 min of ischemia/reoxygenation (I/R alone) or preconditioned (IPreC) with 5 min of ischemia and 5 min of reoxygenation.
