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. 2017 Apr 5;12(4):e0174523. doi: 10.1371/journal.pone.0174523

Fig 3. Dose-dependent inhibitory ability of shRNA plasmids against influenza A/WSN/33 virus in Vero cells.

Fig 3

Vero cells were pretreated with shRNA, which was followed by infection with influenza A/WSN/33 virus at 48 hours. Cell supernatants were harvested at 24 hours post-infection and subjected to a plaque reduction assay to determine the viral titers. Compared with the green fluorescent protein (GFP) vector control group, PAsh1, PAsh2, and PB2sh2 significantly inhibited virus replication in a dose-dependent pattern and reached a maximal inhibitory effect at 8–10 μg, which depended on the shRNA that was used.