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. 2017 Apr 5;12(4):e0174523. doi: 10.1371/journal.pone.0174523

Fig 4. Inhibitory ability of shRNA treatment in wild type versus mutant PA and PB2 proteins.

Fig 4

Four plasmids, two containing wild-type (WT) and two mutant (mut) viral proteins, individually co-transfected with inhibitory shRNAs. (a) PAsh1-shRNA and PA (WT) plasmids, (b) PAsh1-shRNA and PA (mut) plasmids, (c) PAsh2-shRNA and PA (WT) plasmids, (d) PAsh2-shRNA and PA (mut) plasmids, (e) PB2sh2-shRNA and PB (WT) plasmids, and (f) PB2sh2-shRNA and PB (mut) plasmids. The shRNA inhibitory effects on wild type (WT) PA and PB2 proteins were evident in Western blots, while no inhibitory effects were seen in the mutant (mut) protein expression levels. The knockdown effects of the shRNAs on wild type proteins were concordant with the administered dose.