Fig 5. The effect of temperature and gaseous RONS formation on skin damage.

A) The surface temperature was measured on the mouse skin during the plasma treatment using IR camera. Mice were treated under different flow rate conditions. The maximum temperature caused by plasma treatment is indicated on each image. B) OES spectrum of RONS generated in plasma flow during the skin treatment at the treatment site. C) Intensity of different RONS, measured under different flow rate parameters. D) Combined experimental setup for the purposes of OES measurements. The mice were placed under helium (He) jet and were anesthetized during the procedure. The spectrometer was placed perpendicular to He plasma jet, directed to the treated skin area.