Fig. 1.

CHIP regulates CSC properties by affecting CD166 expression in HNCs. (A) The effect of altered CHIP expression on the microsphere formation ability of HN13 (**p=0.007) and UMSCC12 cells (**p=0.003). (B) The protein levels of CHIP targeting oncogene-related proteins and candidate CSC markers were determined in CHIP^OE HNC cells by immunoblotting assays. CD166 expression was decreased in CHIP^OE HN13 cells (**p=0.0053) and HN30 cells (*p=0.0256). (C) Real-time qPCR analysis was conducted to determine the mRNA level of CHIP and CD166 in HN13 and HN30 cells 24 h after transfection. (D) Immunohistochemistry staining for CD166 and CHIP was conducted using consecutive sections of HNC samples, bar=200 µm. (E) CHIP and CD166 expression levels were examined in consecutive sections of UMSCC12-Control and UMSCC12-shCHIP xenografts by immunohistochemistry staining, bar=100 µm.