Table 1. Features of the CPE strains and their carbapenemase-carrying plasmids.
PCR detection of antimicrobial resistance genes | Characterization of the carbapenemase-carrying plasmids | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Isolate | Species | Sampling date | MLST | Carbapenemases | Oxacillinase | Other resistance genes | Plasmid size | Inc groups | PAS | PPS | |||||||
1-CAR | E. coli | 2014/07/08 | ST1434 | blaKPC-2 | — | — | blaOXA-1 | aac(6')-Ib-cr | qnrB6 | 70 kb | IncN | — | — | vagCD | — | — | — |
2-CAR | E. coli | 2014/07/08 | ST5001 | blaKPC-2 | — | — | — | — | — | 48 kb | — | IncR | — | — | — | — | parAB |
3-CAR | E. cloacae | 2014/05/27 | ST822 | — | blaIMI-2 | — | — | — | — | 170 kb | — | — | IncFIB | — | — | — | — |
4-CAR | E. coli | 2014/07/08 | ST216 | blaKPC-2 | — | — | — | aac(6')-Ib | — | 48 kb | — | IncR | — | — | parDE | ccdAB | — |
5-CAR | R. ornithinolytica | 2014/05/27 | — | — | blaVIM-1 | blaOXA-1 | aac(6')-Ib | qnrB5 | 70 kb | — | IncR | — | vagCD | — | — | — | |
6-CAR | K. pneumoniae | 2014/05/27 | ST634 | blaKPC-2 | — | — | — | — | — | 97 kb | — | — | IncFIIK | vagCD | — | — | — |
7-CAR | E. cloacae | 2014/07/08 | ST823 | blaKPC-2 | — | — | blaOXA-1 | aac(6')-Ib-cr | qnrB6 | 70 kb | IncN | — | — | vagCD | — | — | — |
8-CAR | K. oxytoca | 2014/05/27 | blaKPC-2 | — | blaVIM-1 | blaOXA-1 | aac(6')-Iba | — | 60 kb | IncN | — | — | — | — | — | parAB |
a, This strain was positive for the presence of the aac(6')-Ib gene although it was detected in a different plasmid of circa 240 kb.