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. 2017 Apr 5;12(4):e0175095. doi: 10.1371/journal.pone.0175095

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© 2017 Ahrens et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A Different shapes and sizes of discs were obtainable. Scale bar equals 10 mm. B Discs crosslinked in vapor-phase (white) and solution (gray) were stable over weeks upon incubation in PBS. Both types showed a high degree of swelling in the first hours of incubation due to rehydration. Data is presented as mean ± SD, n = 6. C Degree of crosslinking determined for gelatin crosslinked in the vapor-phase (white) and solution (gray). Data is presented as mean ± SD, n = 6. D SEM micrograph of a vapor-phase crosslinked disc’s interior, revealing a porous structure. Scale bar equals 500 μm. Scale bar in inset (3x) equals 50 μm. E hMSCs cultured in delivery disc-conditioned medium remained viable over 7 days, as assessed using a MTT assay. Data is presented as mean ± SD, n = 3.