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Synthesis of bone minerals by encapsulated stem cells: (A-C) Xylenol orange mineral staining photos of hESC-MSCs (The other two cell types had similar images). (D) Xylenol orange mineral staining area fraction (mean ± sd; n = 6). (E-G) ARS staining images of hUCMSCs in CPC-CAF (The other two cell types had similar images). (H) ARS mineral concentration synthesized by cells in CPC-CAF measured by an osteogenesis assay (mean ± sd; n = 6).
