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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Cell Host Microbe. 2016 Jul 13;20(1):49–59. doi: 10.1016/j.chom.2016.06.003

Table 1.

Kinetic parameters of CdnP on cyclic mono and dinucleotides.

Substrate Km (μM) Kcat (s−1) Kcat/Km (s−1/M−1)
Cyclic nucleotide
2′,3′ cGMP 22 ± 4 9.4 4.2 × 105
2′,3′ cUMP 42 ± 10 19.8 4.7 × 105
3′,5′ cAMP n.d. n.d. n.d.
3′,5′ cGMP n.d. n.d. n.d.
Cyclic dinucleotide
c-di-AMP 14 ± 1 17.4 1.2 × 106
c-di-GMP 13.2 ± 6 2.6 1.9 × 105
c-GAMP 4.0 ± 1.6 4.5 1.1 × 106
2′3′ cGAMP n.d. n.d. n.d.

The Vmax and Km (mean ± S.D.) were obtained from double reciprocal plots of initial velocity measurements with at least five different concentrations of substrates. The kcat was calculated assuming a molecular mass of 81 kDa. (n.d. = substrate hydrolysis not detected.)