Table 1.
List of Mutated amino acids created in mutant Chase ABC relative wild type Chase ABC. Mutation sites in the Chase enzyme engineered a mutant DNA to generate a humanized form of the Chase enzyme (ChaseM). The ChaseM sequence was preceded by a human IgG κ-chain leader sequence to enhance secretion and was five potential N-glycosylation sites were mutated (N282Q, N338Q, N345Q, S517Q, and N675Q).
| Wild Type Chase | Mutant Chase |
|---|---|
| No Leader sequence | IgGκ secretion signal |
| 282N | 282Q |
| 338N | 338Q |
| 345N | 345Q |
| 517N | 517Q |
| 675N | 675Q |