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. 2017 Apr 6;8:578. doi: 10.3389/fmicb.2017.00578

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Copyright © 2017 Sridevi, Sudhakar, Ananthathatmula, Nankar and Doble.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of MtbFtsZ in M. smegmatis (A) untreated and (B) treated with 7-dimethyl-4-methyl coumarin. The above figures are the merged images of the dyes. FtsZ is seen as a green spots all over the cells as seen in the inset and they are not observed in the treated cells. Inset represents the single bacteria of interest where MtbFtsZ is seen clearly in the control cells (A) Scale 5 μM. Images were obtained in Carl – Zeiss microscope fitted with AxioCam 506. The filters used were 493–520 nm for alexa fluor and 358–463 nm for DAPI. The arrows indicate the localization of FtsZ in the control cells which is absent in cells treated with coumarin.