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. 2017 Apr 6;8:486. doi: 10.3389/fpls.2017.00486

FIGURE 2.

FIGURE 2

Morphological response to brassinosteroid (BR) treatment. (A–D) Phenotype of the wild type (left) and dg1 mutant (right) after BR treatment. (E) Statistical analysis of the leaf angle in the wild type and dg1 mutant after BR treatment. (F) expression analysis of BR biosynthetic and signaling genes. 0, 0.01, 0.1, and 1 μM represent a series of BR concentrations. Total RNA was isolated from the leaves of the untreated wild type, the untreated dg1 mutant, the wild type after BR treatment and the dg1 mutant after BR treatment at the seedling stage. WT, wild type. Black asterisk indicates the significant difference of the expression levels of D2, BRD1, OsBRI1, and OsBZR1 between the wild type and dg1 mutant. Red asterisk indicates the significant difference of the decreased range of expressions levels of D2, BRD1, and OsBZR1 between the wild type group and dg1 group. Error bars indicate SD. ∗∗Significant difference at P < 0.01 compared with the wild type by Student’s t-test. ∗Significant difference at P < 0.05 compared with the wild type by Student’s t-test. Bars = 0.5 cm.