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Evidence of nitrosoheme. Nitrosoheme was extracted from nitrosomyoglobin with 80% acetone from supernatants of strains grown in TSB supplemented with metmyoglobin and inoculated under (A) limited oxygenation (no stirring and reaction mixture covered with mineral oil) and (B) aerobic conditions (150 rpm). Curves show spectra representative of three biological replicates.
