Table 1.
List of strains and vectors used in this study.
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Staphylococcus xylosus | ||
| C2a | Derived from the type strain DSM20267 and genetically transformable strain | Götz et al., 1983, LN554884 |
| Δnos | Isogenic mutant of C2a deleted of the SXYL_00923 gene, Δnos::ermB | This study |
| ΔnospRBnos | Δnos complemented with nos | This study |
| ΔnospRBpdt | Δnos complemented with pdt (SXYL_00922) | This study |
| ΔnospRBnospdt | Δnos complemented with nospdt | This study |
| C2apRB473 | C2a containing empty vector pRB473 | This study |
| C2apRB474 | C2a containing empty vector pRB474 | This study |
| ΔnospRB473 | Δnos containing empty vector pRB473 | This study |
| ΔnospRB474 | Δnos containing empty vector pRB474 | This study |
| Escherichia coli | ||
| TOP10 | Competent strain for plasmid transformation | Invitrogen |
| Plasmids | ||
| pBT2 | E. coli-S. xylosus thermosensitive shuttle vector, ApR CmR | Brückner, 1997 |
| pEC4 | pBluescript KS + derivative. Source of ermB gene (EmR). ApR | Brückner, 1997 |
| pRB473 | Shuttle vector ApR and CmR | Brückner, 1992 |
| pRB474 | Shuttle vector ApR and CmR | Brückner, 1992 |
| pBTΔnos | pBT2, SXYL_00923 [13-317]::ermB shuttle vector | This study |
| pRBnos | pRB473 derivate for expression of nos | This study |
| pRBpdt | pRB474 derivate for expression of pdt | This study |
| pRBnospdt | pRB473 derivate for expression of nos and pdt | This study |