Table 2.

Oligonucleotides used in this study.

Name	Primer Sequence (5′ to 3′)a,b	Used for
Up923F1-EcoRI Up923R1-ErmF	CGGAATTCGACGCGTACCλTTCCATT CACAATAGAGAGATGTCACCTGTCGATλGGATTTCGCTTC	Amplification of sequence upstream nos
Dn923F2-ErmR Dn923R2-BamHI	GGTATACTACTGACAGCTTCCAACAGAAGCTACTGGTTGTCCA CGGGATCCGGTGTGTCTGCTTGGACλ	Amplification of sequence downstream nos
ErmF-Up923R1 ErmR-Dn923F2	GAAGCGλTCCTTTATCGACAGGTGACATCTCTCTATTGTG TGGACAACCAGTAGCTTCTGTTGGAAGCTGTCAGTAGTATACC	Amplification of ermB
Up923F3 Dn923R3	TCCTGCTCGCACATTACTTG CGTCAGGTATCTTGTTGCTCA	Control of nos::erm construction
Up923PF5-SalI Dn923R5-EcoRI	ACGCGTCGACGTGCTTGATAGCACATGλAAGGA CGGAATTCGGGTCGTTAATGλTGGACA	Complementation of nos in pRB473
Up923PF5-SalI Dn922-EcoRI	ACGCGTCGACGTGCTTGATAGCACATGλAAGGA CGGAATTCTTGCTCATTAGGTTGTGCTAATTC	Complementation of nos-pdt in pRB473
Up922-SalI Dn922-EcoRI	ACGCGTCGACGTAAGGGGTAACGTCλTGλ CGGAATTCTTGCTCATTAGGTTGTGCTAATTC	Complementation of pdt in pRB474
923F4 922R1	AAGCGλTCCTTTATCGACAC CTAATGGCAGGCCCAATAGA	Co-transcription nos-pdt
923F2 923R2	TGCAGAAGCGTTTGAATTTG GCTTCGATGCAGTGAGATGA	qRT-PCR of nos (SXYL_00923)
2505F 2505R	CGTCATCTTCACGλGTCATATTC CGCTAGTACACATTATTATCCAATAG	qRT-PCR of katA (SXYL_2505)
1551F 1551R	ATTCGTGGATTCGCATλAG AGCTTCTGGTAGTGACGT	qRT-PCR of katB (SXYL_1551)
2533F 2533R	TTCGATCATGAACGTATACCA GTGTCTGGTGAACCTTTAGAG	qRT-PCR of katC (SXYL_2533)
1303F 1303R	CGCAGCAGTAGAAGGAACTG ATGTCCACCGCCATTATTGC	qRT-PCR of the housekeeping gene sod (SXYL_1303) and control of DNA contamination in RNA extracts

^aUnderlined regions represent restriction enzyme sequences.

^bPrimer containing overlapping end (in bold).