Table 3.
Genotypic characteristics of S. aureus isolates from bovine intramammary infections and close human contacts.
| PT | Origin of isolates | Sampling site (no. of isolates) | Spa type* | ST* (CC) | agr type | Antimicrobial resistance genes | Immune evasion cluster genes† | Cytotoxin genes detected† | PTSAg genes detected† |
|---|---|---|---|---|---|---|---|---|---|
| PT1 (major) | Bovine | G (9) | t15533 | ST2992 (CC97) | I | blaZ | chp1, sak | hla, hlb, hld, hlg, hlg2, lukED | |
| I (12) | t15539 | ST2992 (CC97) | I | blaZ | hla, hlb1, hld, hlg, hlg2, lukED | ||||
| PT2 (major) | Bovine | E (29) | t189 | ST97 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED | sec28, sell28 | |
| G (7) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED | ||||||
| Human | E (1) | t189 | ST97 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED | sec, sell | ||
| PT3 | Bovine | I (1) | t15539 | ST2992 (CC97) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | |
| PT4 (minor) | Bovine | I (3) | t15539 | ST2992 (CC97) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | |
| PT5 | Bovine | L (1) | t15536 | ST3538 (CC97) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | |
| PT6 | Bovine | I (1) | t15539 | ST2992 (CC97) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | |
| PT7 (major) | Bovine | A (5) | t15538 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||
| B (12) | I | blaZ | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||||
| C (3) | t730 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||
| D (15) | t1201 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||
| F (12) | t1201 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||
| G (1) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||||
| H (3) | t1201 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||
| I (4) | I | Negative | hla, hlb3, hld, hlg, hlg2, lukED, lukM | ||||||
| L (14) | I | Negative | hla, hlb13, hld, hlg, hlg2, lukED, lukM | ||||||
| Human | D(1) | t1201 | ST352 (CC97) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | |||
| PT8 (minor) | Bovine | B (2) | t693 | ST352 (CC97) | I | blaZ | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||
| G (1) | t015 | ST508 (CC45) | I | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | ||||
| PT9 | Bovine | L (1) | t064 | ST8 (CC8) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | sea |
| Human | L (1) | t064 | ST8 (CC8) | I | blaZ | sak | hla, hld, hlg, hlg2, lukED | sea | |
| PT10 (major) | Bovine | F (10) | t529 | ST351 (CC705) | II | Negative | hla, hlb, hld, hlg, hlg2, lukED, lukM | sec, seg, seli, sell, seln, selu, tst | |
| PT11 (minor) | Human | J (2) | t1476 | ST8 (CC8) | I | blaZ | chp, sak | hla, hld, hlg, hlg2, lukED | selj, selr |
| PT12 | Human | C (1) | t360 | ST15 (CC15) | II | blaZ | chp | eta, hla, hld, hlg, hlg2, lukED | |
| PT13 | Human | F (1) | t581 | ST5 (CC5) | II | blaZ | hla, hlb, hld, hlg, hlg2, lukED | sed, seg, sei, selj selm seln, selo, selr, seu | |
| PT14 | Human | A (1) | t2172 | ST707 (CC707) | III | blaZ | chp, sak | hla, hld, hlg, hlg2, lukED | selk, selq, tst |
| PT15 | Human | J (1) | t7023 | ST45 (CC45) | I | blaZ | chp, sak | hla, hld, hlg | sec, selg, seli, sell, selm, seln, selo, selu |
| PT16 | Human | M (1) | t015 | ST508 (CC45) | I | Negative | chp, sak | hla, hld | sec, seg, seli, sell, selm, selo, tst |
| PT17 (minor) | Human | B (2) | t012 | ST30 (CC30) | III | blaZ | sak | hla, hld | seg |
*
New spa types and sequence type are shown in bold font;
†
In some cases not all of the isolates in the group presented with the indicated genes. Where this occurred the number of isolates positive for the gene is indicated by a superscript; CC, Clonal complex; PT, Pulsotype; PTSAg, pyrogenic toxin superantigen; ST, sequence type.