FIGURE 1.

Schematic representation of the PVY recombinant clone in which transcription factor Rosea1 was expressed between NIb and CP: PVY-Ros1. Lines represent the viral 5′ and 3′ untranslated regions (UTR), and boxes denote viral cistrons P1, HC-Pro, P3, P3N-PIPO, 6K1, CI, 6K2, VPg, NIaPro, NIb and CP, as indicated. Rosea1 (Ros1) is indicated by a red box. The nucleotide and amino acid sequences, added to both ends of Rosea1 to complement the split NIb/CP proteolytic site, are also indicated. Arrowheads indicate the cleavage site. The nucleotides in red correspond to silent mutations to avoid homologous repetitions in the PVY-Ros1sequence.