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Diagnosis of PVY-Ros1 in tobacco plants by RT-PCR. Amplification products from the RNA preparations of three plants mock-inoculated (lanes 1 to 3), and inoculated with PVY-Ros1 (lanes 4 to 6), were separated by electrophoresis in agarose gel and revealed by ethidium bromide staining. Lane 7 contains a DNA marker with sizes in bp on the right. The red arrow points to the amplification product that corresponds to cistron PVY CP (801 bp).
