FIGURE 6.

Potato virus Y-Ros1-mediated anthocyanin production in tobacco plants. (A) Representative upper non-inoculated leaves sampled from plants mechanically inoculated with PVY-Ros1. Pictures were taken at different dpi, as indicated. (B) Absorption spectra of the methanol extracts obtained from lower non-symptomatic (green line) and upper symptomatic (red line) tissue from a PVY-Ros1-infected tobacco plant (12 dpi). (C) Plot of average absorbance at 530 nm versus time post-inoculation of the methanol extracts from the upper non-inoculated tissues of the tobacco plants infected with PVY-Ros1. Error bars represent the standard error median. (D) Identification of cyanidin-3-O-rutinoside in the extracts of PVY-Ros1 infected tobacco tissue by a HPLC separation coupled to MS analysis.