A. Averaged elasticity histograms calculated by averaging the bin values of each single-cell elasticity histogram. Error bars correspond to the standard error of the mean. The distributions show that cells plated on a ▽ present the lowest variability, while the ones plated on a Y the highest. B. Coefficient of variation maps of log (E) maps shown in Fig. 2 of RPE1 cells plated on ▽ (n=16), T (n=14), Y (n=18)-shaped fibronectin micropatterns. Coefficient of variation (CV) maps were calculated by dividing the standard deviation of each pixel by its corresponding average value. The medians of CV maps were 0.10, 0.13, and 0.15 for ▽-, T - and Y-cells respectively.