Table 1. K d values of CID–CTD and RRM–RNA interactions.
Domain | Mutant | Ligand | Kd |
---|---|---|---|
CID | WT | Double-repeat CTD | >1,000 μM |
CID | WT | Double-repeat S2P | 23.4±2.7 μM |
CID | WT | Double-repeat S5P | 83.7±12.6 μM |
CID | WT | Double-repeat S7P | 241.8±28.7 μM |
RRM cleaved | WT | RNA: AUUAGUAAAA | 1.84±0.63 μM |
SUMO-RRM | WT | RNA: AUUAGUAAAA | 1.72±0.06 μM |
SUMO-RRM | R392E | RNA: AUUAGUAAAA | 3.33±0.53 μM |
SUMO-RRM | K402A | RNA: AUUAGUAAAA | 2.49±0.33 μM |
SUMO-RRM | Y404A | RNA: AUUAGUAAAA | unstable protein |
SUMO-RRM | T407A | RNA: AUUAGUAAAA | unstable protein |
SUMO-RRM | R428E | RNA: AUUAGUAAAA | 2.87±0.85 μM |
SUMO-RRM | F445A | RNA: AUUAGUAAAA | >1,000 μM |
SUMO-RRM | K447D/A | RNA: AUUAGUAAAA | unstable protein |
SUMO-RRM | F449A | RNA: AUUAGUAAAA | 1.99±0.19 μM |
SUMO-RRM | R450A | RNA: AUUAGUAAAA | 1.91±0.71 μM |
SUMO-RRM | R472E | RNA: AUUAGUAAAA | 17.8±27.5 μM |
SUMO-RRM | F479A | RNA: AUUAGUAAAA | unstable protein |
SUMO-RRM | D486K/A | RNA: AUUAGUAAAA | unstable protein |
SUMO-RRM | F487A | RNA: AUUAGUAAAA | 25.6±15.7 μM |
SUMO-RRM | S492A | RNA: AUUAGUAAAA | 4.20±0.56 μM |
SUMO-RRM | R504E | RNA: AUUAGUAAAA | 2.37±0.17 μM |