Figure 1. Effects of TNF disruption on myogenic responsiveness and in HF.

HF was induced by left anterior descending coronary artery ligation. Sham-operated mice served as controls. Pressure myography of cremaster skeletal muscle resistance arteries isolated from wild type and Tnf^−/− mice. (a) Myogenic responsiveness and (b) phenylephrine-induced vasoconstriction was assessed in arteries from wild-type mice in the absence and presence of etanercept (ETN, 300 μg ml⁻¹ in vitro). (c) Myogenic responsiveness and (d) phenylephrine-induced vasoconstriction was assessed in arteries from Tnf^−/− mice. Numbers in parentheses indicate the number of arteries in each group. All data are mean±s.e.m. *P<0.05 for one-way ANOVA and Dunnett's post hoc test relative to sham (a). An unpaired Student's t-test at each pressure and drug dose was nonsignificant (b–d). ANOVA, analysis of variance.