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. 2017 Apr 4;8:14912. doi: 10.1038/ncomms14912

Figure 6. Cerebellar output function is abnormal in dystonic Ptf1aCre;Vglut2fx/fx mice.

Figure 6

(a) A schematic example of a single-unit extracellular recording in the cerebellum of an awake mouse. (b) A schematic example of the electrode position relative to the neurons of the cerebellar nuclei (CN). (c) WGA-Alexa 555 injection marking the interposed cerebellar nucleus (In). In this example the medial portion of the In was recorded, although in other recordings more lateral In cells were also examined. The fastigial nucleus (Fn), which is the most medially located cerebellar nucleus, is also shown. The surrounding vermis lobules are indicated by Roman numerals. Scale bar 200 μm. (d) Low power examples of P19–P21 juvenile Vglut2fx/fx and Ptf1aCre;Vglut2fx/fx cerebellar nuclei neural firing patterns by binning instantaneous firing rate at 400 ms. (e) High power traces from neurons in the cerebellar nuclei of juvenile Vglut2fx/fx and Ptf1aCre;Vglut2fx/fx mice. Scale bar, 10 ms. (f) Quantifications of juvenile cerebellar nuclear neuron frequency (P=1.35 × 10−2; Student's unpaired t-test), CV (P=0.98; Student's unpaired t-test) and CV2 (P=0.053; Student's unpaired t-test); n=8 Vglut2fx/fx cells (from 3 mice), 11 Ptf1aCre;Vglut2fx/fx cells (from 3 mice). Error bars are defined as s.e.m. ***P<0.001. (g) Low power examples of adult Vglut2fx/fx and Ptf1aCre;Vglut2fx/fx cerebellar nuclei neural firing patterns showing instantaneous firing rate binned at 400 ms. (h) High power traces of neurons of the cerebellar nuclei in adult Vglut2fx/fx and Ptf1aCre;Vglut2fx/fx mice. Scale bar, 10 ms. (i) Quantifications of adult cerebellar nuclear neuron frequency (P=3.594 × 10−5; Student's unpaired t-test), CV (P=5.6 × 10−5; Student's unpaired t-test) and CV2 (P=6.56 × 10−4; Student's unpaired t-test); n=33 Vglut2fx/fx cells (from 14 mice), 27 Ptf1aCre;Vglut2fx/fx cells (from 10 mice). Error bars are defined as s.e.m.