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Reversed-phase HPLC of the crude venom of O. hannah. The fraction corresponding to peptides with a molecular mass between 3 and 10 kDa was applied to a semipreparative Vydac C18 column. 1, 2, 3, and 4 correspond to fractions eluted at 36–39 (fraction 1), 40–43 (fraction 2), 44–46 (fraction 3), and 47–49 (fraction 4) minutes, respectively.
