Table 1.
RPA primers and probes designed in this research.
| Name | Sequence (5′ –3′) | Genome location (DQ231511) |
|---|---|---|
| PCV2 RPA Fe1 | ATACCATAACCCAGCCCTTCTCCTACCACTCCCGC | 443–477 |
| PCV2 RPA Fe2 | ATAACCCAGCCCTTCTCCTACCACTCCCGCTACTT | 448–482 |
| PCV2 RPA Fe3 | AAACCTGTCCTAGATTCCACTATTGATTACTTCCA | 490–524 |
| PCV2 RPA Re1 | TTGTATTCCTGGTCGTATATACTGTTTTCGAACGC | 601–635 |
| PCV2 RPA Re2 | ATATTGTATTCCTGGTCGTATATACTGTTTTCGAA | 604–638 |
| PCV2 RPA Re3 | TTACACGGATATTGTATTCCTGGTCGTATATACTG | 612–646 |
| PCV2 RPA Pe | ATTACTTCCAACCAAACAACAAAAGAAATCAGCTG | 515–569 |
| (FAM-dT)G(THF)C(BHQ1-dT)GAGACTACAAACTGC-C3 space | ||
| PCV2 RPA Fn1 | ATACCATAACCCAGCCCTTCTCCTACCACTCCCGC | 443–477 |
| PCV2 RPA Fn2 | ATAACCCAGCCCTTCTCCTACCACTCCCGCTACTT | 448–482 |
| PCV2 RPA Fn3 | AAACCTGTCCTAGATTCCACTATTGATTACTTCCA | 490–524 |
| PCV2 RPA Rn1 | biotin-TTGTATTCCTGGTCGTATATACTGTTTTCGAACGC | 601–635 |
| PCV2 RPA Rn2 | biotin-ATATTGTATTCCTGGTCGTATATACTGTTTTCGAA | 604–638 |
| PCV2 RPA Rn3 | biotin-TTACACGGATATTGTATTCCTGGTCGTATATACTG | 612–646 |
| PCV2 RPA Pn | FAM-TACTTCCAACCAAACAACAAAAGAAATCAGCTGTG | 517–567 |
| -THF-CTGAGACTACAAACT-C3 space |