Table 2.
AC regulated the levels of ROS, MDA, GSH-Px, and SOD in the serum, liver, and skeletal muscle of mice with and without swimming.
| CTRL | AC (0.1 g/kg) | AC (0.3 g/kg) | AC (0.9 g/kg) | |
|---|---|---|---|---|
| Serum | ||||
| Without swimming | ||||
| MDA (nmol/mL) | 0.69 ± 0.07 | 0.47 ± 0.05∗ | 0.5 ± 0.05∗ | 0.58 ± 0.04 |
| GSH-Px (U/mL) | 39.93 ± 3.03 | 45.61 ± 1.97 | 47.07 ± 2.26∗ | 45 ± 2.87 |
| SOD (U/mL) | 33.26 ± 1.91 | 36.21 ± 1.08 | 41.27 ± 1.87∗∗ | 46.36 ± 0.86∗∗∗ |
| With swimming | ||||
| MDA (nmol/mL) | 0.90 ± 0.08∧∧ | 0.65 ± 0.08#∧∧ | 0.67 ± 0.05#∧∧ | 0.80 ± 0.05∧∧ |
| GSH-Px (U/mL) | 42.75 ± 2.14 | 51.64 ± 1.79##∧ | 48.79 ± 1.38# | 45.43 ± 1.22 |
| SOD (U/mL) | 33.13 ± 1.58 | 35.80 ± 1.67 | 39.20 ± 1.85# | 42.93 ± 1.07## |
|
| ||||
| Liver | ||||
| Without swimming | ||||
| ROS (FI/mgprot) | 222.90 ± 22.54 | 156.75 ± 26.84 | 128.11 ± 25.74∗ | 152.00 ± 19.32∗ |
| MDA (nmol/mgprot) | 0.78 ± 0.02 | 0.71 ± 0.03 | 0.68 ± 0.02∗ | 0.71 ± 0.01∗ |
| GSH-Px (U/mgprot) | 290.39 ± 17.32 | 323.8 ± 24.09 | 316.55 ± 13.23 | 322.83 ± 17.3 |
| SOD (U/mgprot) | 7.66 ± 0.41 | 8.4 ± 0.4 | 8.64 ± 0.43 | 8.65 ± 0.25∗ |
| With swimming | ||||
| ROS (FI/mgprot) | 290.67 ± 33.92∧ | 196.83 ± 36.54 | 183.88 ± 22.21#∧ | 187.29 ± 39.29# |
| MDA (nmol/mgprot) | 0.83 ± 0.04 | 0.75 ± 0.03 | 0.74 ± 0.03 | 0.73 ± 0.03# |
| GSH-Px (U/mgprot) | 282.31 ± 17.4 | 335.64 ± 21.31 | 333.72 ± 19.96 | 348.28 ± 18.36# |
| SOD (U/mgprot) | 7.47 ± 0.37 | 8.21 ± 0.38 | 8.29 ± 0.44 | 8.60 ± 0.28# |
|
| ||||
| Skeletal muscle | ||||
| Without swimming | ||||
| ROS (FI/mgprot) | 106.89 ± 6.67 | 95.4 ± 6.18 | 83.1 ± 2.49∗∗ | 90.20 ± 0.71∗ |
| MDA (nmol/mgprot) | 9.63 ± 0.48 | 7.43 ± 0.53∗ | 7.56 ± 0.24∗ | 7.46 ± 0.21∗ |
| GSH-Px (U/mgprot) | 79.42 ± 1.75 | 85.19 ± 4.06 | 90.95 ± 1.95∗ | 91.57 ± 0.88∗∗ |
| SOD (U/mgprot) | 78.04 ± 1.22 | 80.11 ± 2.51 | 84.77 ± 1.43∗ | 83.36 ± 2.93 |
| With swimming | ||||
| ROS (FI/mgprot) | 111.78 ± 5.21 | 96.60 ± 5.18 | 95.88 ± 3.73# | 97.22 ± 3.79#∧ |
| MDA (nmol/mgprot) | 9.13 ± 0.41 | 7.41 ± 0.45# | 7.63 ± 0.33# | 8.39 ± 0.33 |
| GSH-Px (U/mgprot) | 65.52 ± 3.19∧ | 76.61 ± 4.73∧ | 82.05 ± 4.8#∧ | 87.89 ± 6.17# |
| SOD (U/mgprot) | 78.31 ± 1.59 | 93.35 ± 3.5##∧ | 83.56 ± 1.32 | 86.81 ± 1.55# |
The data are expressed as means ± SEM (n = 12) and analyzed using a one-way ANOVA. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with control mice without swimming; #P < 0.05 and ##P < 0.01 compared with control mice with 60 min swimming; ∧P < 0.05 and ∧∧P<0.01 in a comparison between the same agent treated mice with and without 60 min swimming.