Figure 3.

Liver processing for isolation of non‐parenchymal cells. (a) Liver aspect after perfusion in vivo. (b) Initial digestion in a Petri dish using collagenase‐based solution. (c, d) Liver is minced to very small fragments using scissors to enhance digestion by collagenase. (e) Final aspect after digestion protocol. [Colour figure can be viewed at wileyonlinelibrary.com]