Fig. 5.

DNA methylation predictive signature of HPV status in HNSCCs. a Overall and class-specific misclassification errors based on the number of CpGs selected to predict HPV status. b AUC values from PAM algorithm according to the number of probes selected to predict the HPV status. The red dot indicates the AUC for 10-CpG signatures. Similar results were obtained using RF algorithm (data not shown). c Receiver operating characteristic (ROC) curves (AUC) using the training set data. d ROC curves (AUC) using the test set data. e Average methylation index (AMI) of the 5-CpG signature across the different datasets (TCGA cohort, UCL cohort, FITMANET cohort 450 K, FITMANET validation cohort). f AMI of the 5-CpG signature in cervical carcinomas from TCGA. Similar to HNSCCs signature, high methylation is considered when the AMI is higher than 0.75