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. 2017 Apr 5;17:89. doi: 10.1186/s12866-017-0997-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Confocal laser scanning microscopy of frozen section of nasal tissue from an S. aureus carrier. Overview (a-b) and detailed/zoomed portion of image (c-d) showing S. aureus in stratum spinosum. Scale bar is in micrometers. Green fluorescence labeling of S. aureus, blue fluorescence labeling of keratinocyte cell nuclei and S. aureus DNA, red fluorescence labeling of actin. Primary antibody rabbit polyclonal antibody to S. aureus (ab20920, Abcam), secondary antibody Alexa Fluor 488® goat anti-rabbit IgG (Molecular Probe™, Thermo Fisher Scientific), Alexa Fluor 594 Phalloidin (Molecular Probes™, Thermo Fisher Scientific) and DRAQ5 (BioStatus). We used a CLSM ×63 objective