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. 2017 Apr 6;7:44027. doi: 10.1038/srep44027

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(a) Extinction intensity change of GNR-anti-TnT (opened square) and GNR-anti-TnT-PNIPAAM (filled square) vs. anti-logarithm of TnT concentration, (b) UV-NIR spectra of GNR-anti-TnT-PNIPAAM LSPR nano-immunoassay when 1 (blue) and 15 (red) ng/mL TnT solutions were treated at 37 °C, rectangular (c′) zone is expanded to produce (c) left and right boundaries of green zones are the λ_LSPR for 1 and 15 ng/mL TnT solution respectively and (d) λ_LSPR shift vs TnT concentration in the range of from 1 to 15 ng/mL at 37 °C.